GG COVID-19 Real-Time Triplex PCR kit
The GG COVID-19 Triplex Real-Time PCR Kit was designed to detect open reading frame (ORF1ab）and nucleoprotein (N) based on the genomic database of COVID-19. Reverse transcription and RT-PCR are carried out sequentially in the same tube.
All components required for reaction are added during setup, and there is no need to add additional components once the reaction has been started, which is a very fast and will give satisfactory results in most cases. This product contains polymerase, reverse transcriptase, dNTPs, 5x reaction buffer, RNase inhibitor, specific primers and probes for detection except template. You just need add distilled water and template for RT-PCR and its procedure is very simple.
The GG COVID-19 Triplex Real-Time PCR kit consists of ORF1ab and N and a reference gene. In our GG COVID-19 Triplex Real-Time PCR Kit , an internal control human housekeeping gene is amplified from the sample as well as the ORF1ab and N of SARS-CoV-2 in one tube. To obtain simultaneous fluorescent signals, HEX, FAM and TEXAS RED probes were used to label the IC (reference gene) and ORF1ab/N-specific probes, respectively.
|Specimen Type||Respiratory material* (nasopharyngeal and oropharyngeal swab in ambulatory patients and sputum (if produced) and/or endotracheal aspirate or bronchoalveolar lavage in patients with more severe respiratory disease)|
|Kit Contents (2 options)||Specific primer& probe sets, Positive control and Internal control|
|5x One step RT-PCR buffer, dNTP MIX (10mM EACH), One step RT-PCR Enzyme mix ,Specific primer& probe sets, Positive control, Internal Control, and RNase free D.W.|
|Test number per kit||100, 200, 1000|
|Constituents||Purchase option #1||Purchase option #2||Purchase option #3|
|(2) Postive (ORF and N gene of COVID-19) and internal controls||o||o||o|
|(3) Reverse transcription (RT)-PCR premix (one step reverse transciptase/Taq polymerase enzyme, RT-PCR buffer, dNTP, RNase free water)||X||o||o|
|(4) RNA extraction kit||X||x||o|
|Pros||Fastest*||Cheapest||May be convenient|
|Constituents||Less convenient if short on supply**||Fast and cheap||Slowest|
*small in volume, enables fast dhl/fedex shipping from South Korea.
**Have to buy from other distributors and may have to validate other components of testing.