GG COVID-19 Real-Time Triplex PCR kit
Contents
The GG COVID-19 Triplex Real-Time PCR Kit was designed to detect open reading frame (ORF1ab）and nucleoprotein (N) based on the genomic database of COVID-19. Reverse transcription and RT-PCR are carried out sequentially in the same tube.
All components required for reaction are added during setup, and there is no need to add additional components once the reaction has been started, which is a very fast and will give satisfactory results in most cases. This product contains polymerase, reverse transcriptase, dNTPs, 5x reaction buffer, RNase inhibitor, specific primers and probes for detection except template. You just need add distilled water and template for RT-PCR and its procedure is very simple.
The kit uses TaqMan based real-time PCR technique to conduct in vitro reverse transcription of SARS-CoV-2 RNA, DNA amplification and fluorescence detection. The assay targets specific genomic regions of SARS-CoV-2: nucleocapsid (N) gene and ORF1ab. The TaqMan probes for the two amplicons are labeled with FAM and Texas red fluorescent dyes, respectively, to generate target-specific signals. The assay includes an RNA internal control (PBGD) to monitor the processes from nucleic acid extraction to fluorescence detection. The IC probe is labeled with VIC fluorescent dye to differentiate its fluorescent signal from SARS-CoV-2 targets. The kit showed excellent performance (>95% sensitivity and specificity in detection of SARS-COV-2 and LOD of 5 to 100 copies).
During the validation process, the kit showed excellent performance (100% sensitivity and specificity in detection of SARS-COV-2 and LOD of 5 to 100 copies) in clinical and performance evaluation carried out according to FDA EUA guidelines with clinical specimens and materials from BEI Resources (Manassas, VA, USA) and more.
| Specifications | Kit charactersitics |
|---|---|
| Specimen Type | Respiratory material* (nasopharyngeal and oropharyngeal swab in ambulatory patients and sputum (if produced) and/or endotracheal aspirate or bronchoalveolar lavage in patients with more severe respiratory disease) |
| Kit Contents (2 options) | Specific primer& probe sets, Positive control and Internal control |
| 5x One step RT-PCR buffer, dNTP MIX (10mM EACH), One step RT-PCR Enzyme mix ,Specific primer& probe sets, Positive control, Internal Control, and RNase free D.W. | |
| Test number per kit | 100, 200, 1000 |
Kit constituents
| Constituents | Purchase option #1 | Purchase option #2 | Purchase option #3 |
|---|---|---|---|
| (1) Primer/probes | o | o | o |
| (2) Postive (ORF and N gene of COVID-19) and internal controls | o | o | o |
| (3) Reverse transcription (RT)-PCR premix (one step reverse transciptase/Taq polymerase enzyme, RT-PCR buffer, dNTP, RNase free water) | X | o | o |
| (4) RNA extraction kit | X | x | o |
| Pros | Fastest* | Cheapest | May be convenient |
| Constituents | Less convenient if short on supply** | Fast and cheap | Slowest |
*small in volume, enables fast dhl/fedex shipping from South Korea.
**Have to buy from other distributors and may have to validate other components of testing.